BiologyQ1–3 of 3 questions
Page 1 of 1 · English
1
BiologyEasyMCQGSEB · 2025
The Russian species that became extinct in recent years is . . . . . . .
A
Dodo
B
Quagga
C
Thylacine
D
Steller's Sea Cow
Solution
(D) Steller's Sea Cow (Hydrodamalis gigas) was a large marine mammal that was hunted to extinction in the $18^{th}$ century. It was found in the Bering Sea near Russia.
$A$. Dodo was from Mauritius.
$B$. Quagga was from Africa.
$C$. Thylacine was from Australia.
Therefore,the correct option is $D$.
$A$. Dodo was from Mauritius.
$B$. Quagga was from Africa.
$C$. Thylacine was from Australia.
Therefore,the correct option is $D$.
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2
BiologyEasyMCQGSEB · 2025
Which of the following methods is used for the isolation of $DNA$?
A
Selectable marker
B
Micro-injection
C
Biolistic
D
Gel electrophoresis
Solution
(D) The correct answer is $D$.
Gel electrophoresis is a technique used to separate $DNA$ fragments based on their size and charge.
While the question asks for isolation,in the context of standard biotechnology curriculum,gel electrophoresis is the primary method used to isolate and purify specific $DNA$ fragments from a mixture after they have been extracted and digested with restriction enzymes.
Other options like 'Selectable marker' are used for identifying transformants,'Micro-injection' is a method for direct gene transfer,and 'Biolistic' (gene gun) is a method for introducing $DNA$ into cells.
Gel electrophoresis is a technique used to separate $DNA$ fragments based on their size and charge.
While the question asks for isolation,in the context of standard biotechnology curriculum,gel electrophoresis is the primary method used to isolate and purify specific $DNA$ fragments from a mixture after they have been extracted and digested with restriction enzymes.
Other options like 'Selectable marker' are used for identifying transformants,'Micro-injection' is a method for direct gene transfer,and 'Biolistic' (gene gun) is a method for introducing $DNA$ into cells.
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3
BiologyEasyMCQGSEB · 2025
Which of the following is the recognition sequence (target site) for the restriction enzyme $EcoRI$?
A
$5'\, GAGCTC\, 3'$
B
$5'\, GAATTC\, 3'$
C
$5'\, AAGCTT\, 3'$
D
$5'\, GATATC\, 3'$
Solution
(B) The restriction enzyme $EcoRI$ is derived from $Escherichia \text{ } coli \text{ } RY13$.
It recognizes a specific palindromic nucleotide sequence in $DNA$.
The recognition sequence for $EcoRI$ is $5'-GAATTC-3'$ on one strand, and its complementary strand is $3'-CTTAAG-5'$.
Therefore, the correct target range (recognition site) is $5'-GAATTC-3'$.
It recognizes a specific palindromic nucleotide sequence in $DNA$.
The recognition sequence for $EcoRI$ is $5'-GAATTC-3'$ on one strand, and its complementary strand is $3'-CTTAAG-5'$.
Therefore, the correct target range (recognition site) is $5'-GAATTC-3'$.
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