Which of the following is not present in a bioreactor?

$I.$ $DNA$ being a hydrophilic molecule cannot pass through cell membranes.
$II.$ The bacteria should be made competent to accept the $DNA$ molecule.
The correct option regarding the above statements is:

What would happen when one grows a recombinant bacterium in a bioreactor but forgets to add antibiotic to the medium in which the recombinant is growing?

Explain the method for the separation and isolation of $DNA$ fragments.

Which of the following range of temperature is required for denaturation,annealing of primer,and primer extension of $PCR$ respectively?

The optimum temperature for polymerisation in $PCR$ is $....$ while the enzyme responsible for the mentioned step can tolerate temperatures up to $....$. Select the correct option according to the blanks.