What would happen when one grows a recombinan | vedclass

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(B) In the absence of an antibiotic,there is no selective pressure on the recombinant bacteria to retain the plasmid containing the gene of interest.M

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The recombinant bacterium will lose the plasmid.

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(B) In the absence of an antibiotic,there is no selective pressure on the recombinant bacteria to retain the plasmid containing the gene of interest.Maintaining a high copy number of plasmids imposes a metabolic burden on the microbial cells.Consequently,in the absence of antibiotic selection,the bacterial cells will tend to lose the plasmid to conserve energy and improve their growth efficiency.

What would happen when one grows a recombinant bacterium in a bioreactor but forgets to add antibiotic to the medium in which the recombinant is growing?

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