The optimum temperature for polymerisation in | vedclass

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Question

(C) In the Polymerase Chain Reaction $(PCR)$,the extension or polymerisation step is carried out by the enzyme $Taq$ $DNA$ polymerase.The optimum temp

Vedclass · Accepted Answer

$72^{\circ}C, 95^{\circ}C$

Vedclass · Answer

(C) In the Polymerase Chain Reaction $(PCR)$,the extension or polymerisation step is carried out by the enzyme $Taq$ $DNA$ polymerase.The optimum temperature for the activity of $Taq$ $DNA$ polymerase is $72^{\circ}C$.This enzyme is isolated from the thermophilic bacterium $Thermus$ $aquaticus$,which allows it to remain stable and active even at high temperatures,tolerating temperatures up to $95^{\circ}C$ (the denaturation temperature).Therefore,the correct values are $72^{\circ}C$ for polymerisation and $95^{\circ}C$ for enzyme tolerance.

The optimum temperature for polymerisation in $PCR$ is $....$ while the enzyme responsible for the mentioned step can tolerate temperatures up to $....$. Select the correct option according to the blanks.

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