Which of the following range of temperature i | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temper

Vedclass · Accepted Answer

$90-98^{\circ} C, 40-60^{\circ} C, 70-75^{\circ} C$

Vedclass · Answer

(A) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature $(90-98^{\circ} C)$ to separate the strands.$2$. Annealing: The temperature is lowered $(40-60^{\circ} C)$ to allow the primers to bind to the complementary sequences on the single-stranded $DNA$.$3$. Extension: The temperature is adjusted to $70-75^{\circ} C$ to allow the $Taq$ $DNA$ polymerase to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence of temperature ranges is $90-98^{\circ} C, 40-60^{\circ} C, 70-75^{\circ} C$.

Which of the following range of temperature is required for denaturation,annealing of primer,and primer extension of $PCR$ respectively?

Similar Questions

The presence of a chromogenic substrate gives ............ coloured colonies.

In which method is recombinant $DNA$ directly injected into the nucleus of an animal cell?

$PCR$ (Polymerase Chain Reaction) technique is useful in:

Which of the following steps is $NOT$ used in the creation of Genetically Modified Organisms ($GMO$s)?

Which of the following techniques is $NOT$ used for introducing $DNA$ into a host cell in $r-DNA$ technology?

Vedclass Test Series

Exam Paper Generator

Online Exam Module