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(D) To make a bacterial cell competent to take up recombinant $DNA$, it is treated with a specific concentration of a divalent cation, such as $Calciu

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Calcium

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(D) To make a bacterial cell competent to take up recombinant $DNA$, it is treated with a specific concentration of a divalent cation, such as $Calcium$ $(Ca^{2+})$.This treatment increases the efficiency with which $DNA$ enters the bacterium through pores in its cell wall.Following this, the cells are incubated with recombinant $DNA$ on ice, followed by placing them briefly at $42^{\circ}C$ (heat shock), and then putting them back on ice.This enables the bacteria to take up the recombinant $DNA$.

What treatment is given to make a bacterial cell competent to take up $DNA$?

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