Which chemical is utilised during the isolation of $DNA$ at the end of precipitation?

  • A
    Chilled methanol
  • B
    Chilled butanol
  • C
    Chilled ethanol
  • D
    Chilled phenol

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Which of the following steps are involved in the process of recombinant biotechnology? Arrange in correct order.
$I.$ Extraction of the desired gene product
$II.$ Amplification of the gene of interest
$III.$ Isolation of a desired $DNA$ fragment
$IV.$ Ligation of the $DNA$ fragment into a vector
$V.$ Insertion of recombinant $DNA$ into the host
Correct order is:

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