Which of the following is the correct sequence of steps in the Polymerase Chain Reaction $(PCR)$?

Given below are four statements pertaining to the separation of $DNA$ fragments using gel electrophoresis. Identify the incorrect statements:
$(a)$ $DNA$ is a negatively charged molecule and so it is loaded on the gel towards the Anode terminal.
$(b)$ $DNA$ fragments travel along the surface of the gel,whose concentration does not affect the movement of $DNA$.
$(c)$ The smaller the size of the $DNA$ fragment,the larger is the distance it travels through it.
$(d)$ Pure $DNA$ can be visualized directly by exposing it to $UV$ radiation.
Choose the correct answer from the options given below:

Consider the following figure of a stirred-tank bioreactor and identify $A, B, C$,and $D$.

In which of the following methods is a probe allowed to hybridize to its complementary $DNA$ in a clone of cells?

In the case of polymerase chain reaction $(PCR)$,what are the temperatures required for the following steps?
$A.$ Denaturation
$B.$ Annealing
$C.$ Extension

Following enzymes/chemical/technique are used in the process of gel electrophoresis:
$I.$ sample $DNA$ is cut into fragments
$II.$ restriction endonucleases
$III.$ agarose gel
$IV.$ ethidium bromide
$V.$ $UV$-radiation
$VI.$ elution
Mark the correct sequence of their use.