Which one of the following statements is wron | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(D) The correct answer is $D$. The statement 'The smaller $DNA$ fragments separate first' is technically incorrect in the context of the final separat

Vedclass · Accepted Answer

The smaller $DNA$ fragments separate first.

Vedclass · Answer

(D) The correct answer is $D$. The statement 'The smaller $DNA$ fragments separate first' is technically incorrect in the context of the final separation pattern,as they migrate the fastest and reach the farthest point,but they do not 'separate first' in a chronological sense; rather,they are the ones that travel the furthest distance through the gel matrix.Gel electrophoresis is a technique that uses an electric field to separate $DNA$ molecules based on their size.Since $DNA$ molecules are negatively charged due to the phosphate backbone,they migrate towards the positive electrode (anode).The rate of migration is inversely proportional to the size of the $DNA$ fragment.Smaller $DNA$ fragments encounter less resistance from the agarose matrix and thus migrate faster and travel further than larger fragments.Therefore,the band located farthest from the loading well represents the smallest $DNA$ fragment.

Which one of the following statements is wrong with respect to separation of $DNA$ fragments on gel electrophoresis?

Similar Questions

Which of the following diagrams is correct for primer annealing in $PCR$?

Select the correct sequence of steps in $PCR$.

It is used to detect $HIV$ in suspected $AIDS$ patient and detect mutations in genes in suspected cancer patient too.

During the purification process for recombinant $DNA$ technology,the addition of chilled ethanol precipitates out:

In the presence of a chromogenic substrate,what color colonies do recombinants and non-recombinants produce,respectively?

Vedclass Test Series

Exam Paper Generator

Online Exam Module