Which of the following diagrams is correct fo | vedclass

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(B) In the Polymerase Chain Reaction $(PCR)$,primers are short,single-stranded $DNA$ sequences that are complementary to the flanking regions of the t

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(B) In the Polymerase Chain Reaction $(PCR)$,primers are short,single-stranded $DNA$ sequences that are complementary to the flanking regions of the target $DNA$ sequence.$1$. $DNA$ strands are antiparallel,meaning one strand runs in the $5' \rightarrow 3'$ direction and the other in the $3' \rightarrow 5'$ direction.$2$. Primers anneal to the template $DNA$ strands such that they are complementary and antiparallel to the template.$3$. Specifically,the primer must bind to the $3'$ end of the template strand so that $DNA$ polymerase can extend it in the $5' \rightarrow 3'$ direction.$4$. In diagram $B$,the primers are correctly oriented: the primer on the top strand (which is $5' \rightarrow 3'$) binds to the $3'$ end of the template,and the primer on the bottom strand (which is $3' \rightarrow 5'$) binds to the $3'$ end of its respective template,allowing for proper extension.Therefore,the correct diagram is $B$.

Which of the following diagrams is correct for primer annealing in $PCR$?

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