Which one of the following pairs is not correctly matched?

Match the following:-

Column-$I$	Column-$II$
$(A)$ $\text{YAC}$	$(I)$ Separation of $\text{DNA}$ fragments
$(B)$ $\text{Hind II}$	$(II)$ Yeast
$(C)$ Gel electrophoresis	$(III)$ First restriction endonuclease
$(D)$ Heterologous host	$(IV)$ Recombinant protein

What do '$X$' and '$Y$' represent in the given diagram of $pBR322$?

Assertion $:-$ The tumor inducing $(Ti)$ plasmid of $Agrobacterium$ $tumefaciens$ has now been modified into a cloning vector.
Reason $:-$ Retroviruses have been disarmed and are now used to deliver genes into animal cells.

Read the following statements and select the incorrect one:
$(I.)$ Disarmed Agrobacterium is used to deliver desirable gene into plant cell.
$(II.)$ In $\text{EcoRI}$,the letter '$R$' is derived from the name of species.
$(III.)$ Gene therapy is a part of modern biotechnology.
$(IV.)$ $\text{DNA}$ is a hydrophilic molecule,so it can easily pass through cell membrane.

Choose the incorrect option $-$