What do '$X$' and '$Y$' represent in the given diagram of $pBR322$?

Match the following items in List-$I$ with List-$II$:

List-$I$	List-$II$
$(a)$ Gel electrophoresis	$(1)$ Amplification of gene of interest
$(b)$ $PCR$	$(2)$ Separation of $DNA$ fragments
$(c)$ Bioreactor	$(3)$ Plasmid
$(d)$ Cloning vector	$(4)$ Production of desired product

Given below are two statements: one is labelled as Assertion $(A)$ and the other is labelled as Reason $(R)$.
Assertion $(A):$
Polymerase chain reaction is used in $DNA$ amplification.
Reason $(R):$
The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,choose the correct answer from the options given below:

Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.

Match List-$I$ with List-$II$:

List-$I$	List-$II$
$A$. Genetically modified organism	$I$. Agrobacterium tumefaciens
$B$. $DNA$ polymerase	$II$. Bt cotton
$C$. Ti plasmid	$III$. Thermus aquaticus
$D$. pBR322	$IV$. Escherichia coli

Choose the correct answer from the options given below:

Identify '$Z$' and '$Q$' in the given figure of $pBR322$.