Manipulation of DNA in genetic engineering be | vedclass

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(A) The isolation of restriction endonucleases by Nathans and Smith $(1970)$ made it possible to cut $DNA$ at specific sites.Restriction enzymes can c

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Restriction endonuclease

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(A) The isolation of restriction endonucleases by Nathans and Smith $(1970)$ made it possible to cut $DNA$ at specific sites.Restriction enzymes can cut both strands of $DNA$ when foreign nucleotides are introduced into the cell.They cleave $DNA$ to generate a nick with a $5'$ phosphoryl and $3'$ hydroxyl terminus,which is the fundamental step in recombinant $DNA$ technology.

Manipulation of $DNA$ in genetic engineering became possible due to the discovery of

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Which statement is correct for the $tet^R$ gene in the $pBR322$ plasmid?

Which enzyme is used as molecular scissors?

In the $pBR322$ plasmid,the ampicillin and tetracycline resistance genes contain recognition sites for how many restriction enzymes,respectively?

Assertion : Plasmids are extrachromosomal $DNA$.
Reason : Plasmids are found in bacteria and are useful in genetic engineering.

The process of replication in plasmid $DNA$,other than initiation,is controlled by

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