In the pBR322 plasmid,the ampicillin and tetr | vedclass

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Question

(A) The $pBR322$ plasmid is a widely used cloning vector in biotechnology.It contains two antibiotic resistance genes: the ampicillin resistance gene

Vedclass · Accepted Answer

$2, 2$

Vedclass · Answer

(A) The $pBR322$ plasmid is a widely used cloning vector in biotechnology.It contains two antibiotic resistance genes: the ampicillin resistance gene $(amp^R)$ and the tetracycline resistance gene $(tet^R)$.The ampicillin resistance gene $(amp^R)$ contains recognition sites for two restriction enzymes: $PstI$ and $PvuI$.The tetracycline resistance gene $(tet^R)$ contains recognition sites for two restriction enzymes: $BamHI$ and $SalI$.Therefore,the number of restriction sites for ampicillin and tetracycline resistance genes are $2$ and $2$,respectively.

In the $pBR322$ plasmid,the ampicillin and tetracycline resistance genes contain recognition sites for how many restriction enzymes,respectively?

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