Which statement is correct for the $tet^R$ gene in the $pBR322$ plasmid?

  • A
    It has a $Pst I$ recognition site.
  • B
    It has $BamH I$ and $Sal I$ recognition sites.
  • C
    It has a $tet II$ recognition site.
  • D
    It has a $Pvu I$ recognition site.

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What is the ampicillin resistance gene?

Which of the following statements is not correct regarding the $Eco RI$ restriction endonuclease enzyme?
$I.$ $Eco RI$ restriction endonuclease enzyme is isolated from $Escherichia \; coli \; RY13$.
$II.$ Its recognition sequence is $5^{\prime}-GAATTC-3^{\prime}$ / $3^{\prime}-CTTAAG-5^{\prime}$.
$III.$ Its site of cleavage is between $G$ and $A$ in the sequence $5^{\prime}-GAATTC-3^{\prime}$.

Plasmids are suitable vectors for gene cloning because

$EcoRI$ derives from ..........

Restriction endonucleases are enzymes that:

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