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(A) The cloning vector $pBR322$ is a widely used plasmid in biotechnology. In the standard map of $pBR322$,the restriction site for $BamHI$ is located

Vedclass · Accepted Answer

$X = BamHI, Y = PstI$

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(A) The cloning vector $pBR322$ is a widely used plasmid in biotechnology. In the standard map of $pBR322$,the restriction site for $BamHI$ is located within the $tet^R$ (tetracycline resistance) gene,and the restriction site for $PstI$ is located within the $amp^R$ (ampicillin resistance) gene. Based on the standard diagram of $pBR322$,the label $X$ points to the $BamHI$ restriction site,and the label $Y$ points to the $PstI$ restriction site. Therefore,the correct identification is $X = BamHI$ and $Y = PstI$.

Identify $X$ and $Y$ from the given figure of the cloning vector $pBR322$.

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