Class 12BiologyBiotechnology Principals and ProcessMix Examples - Biotechnology Principals and Process
MediumGive differences between $PCR$ and plasmid.
(N/A)
| $PCR$ | Plasmid |
|---|---|
| $(1)$ It is an abiotic method of $DNA$ amplification. | $(1)$ It is a biotic vector molecule used for cloning. |
| $(2)$ $PCR$ can generate millions of copies of specific $DNA$ fragments in a short time. | $(2)$ Being a biotic system,the number of copies obtained is generally lower and depends on the host cell replication. |
| $(3)$ $PCR$ is an in vitro process used to amplify specific $DNA$ sequences. | $(3)$ Plasmids are used as vectors to introduce and replicate desired $DNA$ fragments within a living host cell. |
| $(4)$ The process involves cyclic steps: denaturation,annealing,and extension. | $(4)$ Plasmid-based cloning involves restriction digestion,ligation,and transformation,not cyclic $PCR$ steps. |
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Similar Questions
Match the following:-
Column-$I$ Column-$II$ $(A)$ $\text{YAC}$ $(I)$ Separation of $\text{DNA}$ fragments $(B)$ $\text{Hind II}$ $(II)$ Yeast $(C)$ Gel electrophoresis $(III)$ First restriction endonuclease $(D)$ Heterologous host $(IV)$ Recombinant protein
| Column-$I$ | Column-$II$ |
|---|---|
| $(A)$ $\text{YAC}$ | $(I)$ Separation of $\text{DNA}$ fragments |
| $(B)$ $\text{Hind II}$ | $(II)$ Yeast |
| $(C)$ Gel electrophoresis | $(III)$ First restriction endonuclease |
| $(D)$ Heterologous host | $(IV)$ Recombinant protein |
Medium
View SolutionRead the following statements and select the incorrect one:
$(I.)$ Disarmed Agrobacterium is used to deliver desirable gene into plant cell.
$(II.)$ In $\text{EcoRI}$,the letter '$R$' is derived from the name of species.
$(III.)$ Gene therapy is a part of modern biotechnology.
$(IV.)$ $\text{DNA}$ is a hydrophilic molecule,so it can easily pass through cell membrane.
$(I.)$ Disarmed Agrobacterium is used to deliver desirable gene into plant cell.
$(II.)$ In $\text{EcoRI}$,the letter '$R$' is derived from the name of species.
$(III.)$ Gene therapy is a part of modern biotechnology.
$(IV.)$ $\text{DNA}$ is a hydrophilic molecule,so it can easily pass through cell membrane.
Medium
View SolutionMatch the columns:
Column-$I$ Column-$II$ $(a)$ Ethidium Bromide $(i)$ Agrobacterium $(b)$ Ti plasmid $(ii)$ Tetracycline $(c)$ $PvuI$ $(iii)$ Gel electrophoresis $(iv)$ Ampicillin
| Column-$I$ | Column-$II$ |
| $(a)$ Ethidium Bromide | $(i)$ Agrobacterium |
| $(b)$ Ti plasmid | $(ii)$ Tetracycline |
| $(c)$ $PvuI$ | $(iii)$ Gel electrophoresis |
| $(iv)$ Ampicillin |
Medium
View Solution'Cloning' is meant for
Medium
View SolutionAssertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.
MediumAIIMS 2015
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