For proving the semi-conservative mode of rep | vedclass

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(C) The semi-conservative mode of $DNA$ replication was experimentally proven by Matthew Meselson and Franklin Stahl in $1958$ using $E. coli$.They fi

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$^{15}NH_4Cl$

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(C) The semi-conservative mode of $DNA$ replication was experimentally proven by Matthew Meselson and Franklin Stahl in $1958$ using $E. coli$.They first grew $E. coli$ in a medium containing $^{15}N$ (as $^{15}NH_4Cl$) for many generations.This resulted in the incorporation of the heavy isotope $^{15}N$ into the newly synthesized $DNA$ molecules of the bacteria.After this,the cells were transferred into a medium with normal $^{14}N$ (as $^{14}NH_4Cl$) and allowed to divide.By analyzing the density of the $DNA$ using cesium chloride $(CsCl)$ density gradient centrifugation,they confirmed the semi-conservative nature of replication.

For proving the semi-conservative mode of replication of $DNA$,$E. coli$ were first grown in a medium containing?

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