Which of the following did Meselson and Stahl | vedclass

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(D) Meselson and Stahl performed an experiment in $1958$ to prove that $DNA$ replication is semiconservative.They cultured $E. coli$ in a medium conta

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Heavy isotope $^{15}N$ and $E$. coli

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(D) Meselson and Stahl performed an experiment in $1958$ to prove that $DNA$ replication is semiconservative.They cultured $E. coli$ in a medium containing $^{15}NH_4Cl$ ($^{15}N$ is the heavy isotope of nitrogen) as the only nitrogen source for many generations.This resulted in the incorporation of $^{15}N$ into the newly synthesized $DNA$.Subsequently,they transferred the cells into a medium with normal $^{14}NH_4Cl$ and took samples at various definite time intervals as the cells multiplied.The extracted DNAs were centrifuged in a cesium chloride $(CsCl)$ density gradient to measure the densities of $DNA$.Thus,they used the heavy isotope $^{15}N$ and $E. coli$ bacteria for their experiment.

Which of the following did Meselson and Stahl use in the experiment to prove semiconservative $DNA$ replication?

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