(N/A) The process of cutting $DNA$ involves the following steps:
$1$. Restriction enzyme digestions are performed by incubating purified $DNA$ molecules with specific restriction enzymes under optimal conditions (temperature,$pH$,and buffer).
$2$. Agarose gel electrophoresis is employed to check the progression and completion of the restriction enzyme digestion.
$3$. Since $DNA$ is a negatively charged molecule,it moves towards the positive electrode (anode) during electrophoresis.
$4$. The same procedure is repeated with the vector $DNA$ using the same restriction enzyme to ensure complementary sticky ends.
$5$. Finally,the cut 'gene of interest' from the source $DNA$ and the linearized vector $DNA$ are mixed,and the enzyme $DNA$ ligase is added to join them,resulting in the formation of recombinant $DNA$.