Which of the following has popularized the $PCR$ (polymerase chain reaction)?

  • A
    Easy availability of $DNA$ template
  • B
    Availability of synthetic primers
  • C
    Availability of cheap deoxyribonucleotides
  • D
    Availability of thermostable $DNA$ polymerase

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$A$. Ability to multiply and express outside the host in a bioreactor.
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Two enzymes responsible for restricting the growth of bacteriophages in $E. coli$ were isolated. One was methylase and the other was restriction endonuclease. What is the significance of methylase?

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