The Polymerase Chain Reaction $(PCR)$ technique is used for ........

The optimum temperature for polymerisation in $PCR$ is $....$ while the enzyme responsible for the mentioned step can tolerate temperatures up to $....$. Select the correct option according to the blanks.

Significance of treating bacterial cells with calcium chloride before transformation is to facilitate

Give the various methods to make bacteria a competent host.

In this technique,large quantities of biotechnological products such as antibiotics,vaccines,and enzymes are produced by maintaining and growing only the desired microbes/eukaryotic cells in a sterile environment.

The technique of bombarding plant cells with high velocity microparticles of gold or tungsten,coated with $DNA$,is known as: