Insertional inactivation is performed by whic | vedclass

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(C) Insertional inactivation is a technique used to identify recombinant clones. In this process,a foreign $DNA$ fragment is inserted into a gene,whic

Vedclass · Accepted Answer

$\beta$-galactosidase

Vedclass · Answer

(C) Insertional inactivation is a technique used to identify recombinant clones. In this process,a foreign $DNA$ fragment is inserted into a gene,which results in the inactivation of that gene. For example,the gene encoding the enzyme $\beta$-galactosidase can be used as a selectable marker. If a foreign $DNA$ is inserted into the coding sequence of the $\beta$-galactosidase gene,the enzyme becomes inactivated. Consequently,the colonies do not produce any color in the presence of a chromogenic substrate,allowing for the identification of recombinant colonies.

Insertional inactivation is performed by which enzyme?

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