Which of the following statements is $NOT$ correct?

  • A
    Insulin in humans is synthesized as proinsulin.
  • B
    Proinsulin contains an extra peptide called the $C$-peptide.
  • C
    Active insulin consists of $A$ and $B$ chains linked together by hydrogen bonds.
  • D
    Genetically engineered insulin is produced in $E. coli$.

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Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.

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