You have identified a useful gene in bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.
After identifying a useful gene in bacteria, following steps should be undertaken:
$(i)$ Isolation of useful gene using Restriction Endonucleases
$(ii)$ Transferring the gene to a suitable vector to create a recombinant $DNA$ molecule
$(iii)$Transfer of these recombinant $DNA$ molecules to the target cells
$(iv)$ Screening of cells for transformation
$(v)$ Selection of transformed cells
$(vi)$ Regeneration of plants from the transformed cells to get transgenic plants.
Define transgenic animals. Explain in detail any four areas where they can be utilised.
Match the following columns and select the correct option.
Column $- I$ | Column $- II$ |
$(a)$ Bt cotton | $(i)$ Gene therapy |
$(b)$ Adenosine deaminase deficiency | $(ii)$ Cellular defence |
$(c)$ $RNAi$ | $(iii)$ Detection of $HIV$ infection |
$(d)$ $PC R$ | $(iv)$ Bacillus thuringiensis |
$(a)\quad(b)\quad(c)\quad(d)$
Which ingredient was present in higher concentrations in $GM$ rice as compared to the usual rice?
cry $II$ Ab and cry $I$ Ab produce toxins that control
A probe which is a molecule used to locate specific sequences in a mixture of $DNA$ or $RNA$ molecules could be