Two enzymes responsible for restricting the growth of bacteriophages in $E. coli$ were isolated. One was methylase and the other was restriction endonuclease. What is the significance of methylase?

  • A
    Protection of host $DNA$ from the action of restriction endonuclease by adding methyl group to one or two bases usually within the sequence recognized by the restriction enzyme.
  • B
    Able to ligate the two cohesive ends of $DNA$ molecule.
  • C
    Able to remove the methyl group and hence,prevent the action of restriction endonuclease on host $DNA$.
  • D
    Able to cut the $DNA$ of bacteriophage at specific sites.

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The enzyme that is used in $PCR$ technology is:

The function of the 'ori' site in a vector is to

When foreign $DNA$ and a plasmid are cut by the same restriction endonuclease,which enzyme is used to join them to form a recombinant plasmid?

Match the enzymes in Column-$I$ with their respective functions in Column-$II$.
Column-$I$ Column-$II$
$P$. Restriction endonuclease $I$. Synthesizes $DNA$ copies
$Q$. $DNA$ polymerase $II$. Cleaves $DNA$ molecules
$R$. $DNA$ ligase $III$. Joins $DNA$ molecules

Recombinant $DNA$ has an integrated fragment of:

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