Identify the correct sequence of steps in the | vedclass

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(C) The Polymerase Chain Reaction $(PCR)$ consists of three main steps performed in a cyclic manner:$1$. Denaturation: The double-stranded $DNA$ is he

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Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension

Vedclass · Answer

(C) The Polymerase Chain Reaction $(PCR)$ consists of three main steps performed in a cyclic manner:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx. $94-98^{\circ}C$) to separate the strands.$2$. Annealing: The temperature is lowered (approx. $50-65^{\circ}C$) to allow primers to bind to the complementary sequences on the single-stranded $DNA$ templates.$3$. Extension: The temperature is adjusted (approx. $72^{\circ}C$) for the $DNA$ polymerase enzyme to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence is Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension.

Identify the correct sequence of steps in the Polymerase Chain Reaction $(PCR)$.

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