The steps involved in the Southern blot test | vedclass

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(A) The correct sequence of steps in the Southern blotting technique is as follows:$1$. Digestion of $DNA$ with restriction enzymes $(III)$$2$. Separa

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$III, II, IV, V, I$

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(A) The correct sequence of steps in the Southern blotting technique is as follows:$1$. Digestion of $DNA$ with restriction enzymes $(III)$$2$. Separation of $DNA$ fragments by gel electrophoresis $(II)$$3$. Staining with ethidium bromide to visualize $DNA$ under $UV$ light $(IV)$$4$. Hybridization with a radioactive probe $(V)$$5$. Detection using $X-ray$ film (autoradiography) $(I)$Therefore,the correct order is $III, II, IV, V, I$.

The steps involved in the Southern blot test are as follows:
$I.$ $X-ray$ film
$II.$ Electrophoresis
$III.$ Digestion with restriction enzyme
$IV.$ Ethidium bromide
$V.$ Radioactive probe
Choose the option having the correct sequential order of these events.

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The steps involved in the Southern blot test are as follows:$I.$ $X-ray$ film$II.$ Electrophoresis$III.$ Digestion with restriction enzyme$IV.$ Ethidium bromide$V.$ Radioactive probeChoose the option having the correct sequential order of these events.