Identify the correct sequence of steps in eac | vedclass

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Question

(B) The Polymerase Chain Reaction $(PCR)$ consists of three main steps performed in a cycle:$1$. Denaturation: The reaction mixture is heated to $94-9

Vedclass · Accepted Answer

$(2)$ Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension

Vedclass · Answer

(B) The Polymerase Chain Reaction $(PCR)$ consists of three main steps performed in a cycle:$1$. Denaturation: The reaction mixture is heated to $94-98^\circ C$ to separate the double-stranded $DNA$ into single strands.$2$. Annealing: The mixture is cooled to $50-65^\circ C$,allowing the primers to bind to their complementary sequences on the single-stranded $DNA$ templates.$3$. Extension: The temperature is raised to approximately $72^\circ C$,where the $Taq$ $DNA$ polymerase enzyme synthesizes new $DNA$ strands by adding nucleotides to the primers.Thus,the correct sequence is Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension.

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction $(PCR)$:

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