The Polymerase Chain Reaction $(PCR)$ is a reaction in which amplification of specific $DNA$ sequences is carried out in vitro. This statement is

In the above represented plasmid,an alien piece of $\text{DNA}$ is inserted at the $\text{EcoRI}$ site. Which of the following strategies will be chosen to select the recombinant colonies?

Provide definitions/explanations for: Cloning,Elution,and Insertional Inactivation.

When $DNA$ fragments are stained with ethidium bromide and viewed under $UV$ light,what color do they appear?

Given below are two statements:
Statement $I:$ The $\text{DNA}$ fragments extracted from gel electrophoresis can be used in the construction of recombinant $\text{DNA}$.
Statement $II:$ Smaller size $\text{DNA}$ fragments are observed near the anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements,choose the most appropriate answer from the options given below:

In which step of $PCR$ do the primers anneal to the $DNA$ template?