The figure below shows three steps (A, B, C) | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The figure illustrates the process of recombinant $	ext{DNA}$ technology.Step $(A)$ shows the action of a restriction endonuclease (specifically

Vedclass · Accepted Answer

$(C)$ Joining of vector $	ext{DNA}$ and foreign $	ext{DNA}$ with the help of $	ext{DNA}$ ligase

Vedclass · Answer

(A) The figure illustrates the process of recombinant $	ext{DNA}$ technology.Step $(A)$ shows the action of a restriction endonuclease (specifically $	ext{EcoRI}$) on both the vector $	ext{DNA}$ and foreign $	ext{DNA}$ at specific palindromic sequences $(GAATTC)$,resulting in sticky ends.Step $(B)$ shows the base pairing of the complementary sticky ends of the vector $	ext{DNA}$ and foreign $	ext{DNA}$.Step $(C)$ shows the sealing of the nicks in the sugar-phosphate backbone by the enzyme $	ext{DNA}$ ligase,resulting in the formation of recombinant $	ext{DNA}$.Therefore,the correct identification is $(C)$ Joining of vector $	ext{DNA}$ and foreign $	ext{DNA}$ with the help of $	ext{DNA}$ ligase.

The figure below shows three steps $(A, B, C)$ of a process. Select the option giving the correct identification $:-$

Similar Questions

You have identified a useful gene in bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.

In which method is recombinant $DNA$ directly injected into the nucleus of an animal cell?

Which of the following steps is $NOT$ used in the creation of Genetically Modified Organisms ($GMO$s)?

What type of method is the $PCR$ technique?

In gel electrophoresis,the separated bands of $DNA$ are cut out and extracted from the gel piece. This step is called

Vedclass Test Series

Exam Paper Generator

Online Exam Module