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(C) restriction endonucleases and ligases.The enzymes absolutely necessary for recombinant $DNA$ technology are restriction endonucleases and ligases.

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restriction endonucleases and ligases

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(C) restriction endonucleases and ligases.The enzymes absolutely necessary for recombinant $DNA$ technology are restriction endonucleases and ligases.$1$. Restriction endonucleases act as molecular scissors that cut $DNA$ at specific recognition sequences.$2$. Ligases act as molecular glue that joins $DNA$ fragments together to form recombinant $DNA$ molecules.$3$. Topoisomerases are involved in relieving $DNA$ supercoiling but are not the primary tools for recombination.$4$. Polymerases are used for $DNA$ amplification $(PCR)$ but are distinct from the cutting and joining enzymes required for basic recombinant construction.$5$. Peptidases are enzymes that break down proteins and have no role in $DNA$ manipulation.

The enzymes which are absolutely necessary for recombinant $DNA$ technology are

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