Ligation of foreign text{DNA} at which of the | vedclass

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(D) The plasmid $	ext{pBR}^{322}$ contains two antibiotic resistance genes: ampicillin resistance $(amp^R)$ and tetracycline resistance $(tet^R)$.Res

Vedclass · Accepted Answer

Sal $I$

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(D) The plasmid $	ext{pBR}^{322}$ contains two antibiotic resistance genes: ampicillin resistance $(amp^R)$ and tetracycline resistance $(tet^R)$.Restriction sites for $Pst I$ and $Pvu I$ are located within the ampicillin resistance gene $(amp^R)$.Restriction sites for $BamHI$ and $Sal I$ are located within the tetracycline resistance gene $(tet^R)$.Therefore,if foreign $	ext{DNA}$ is ligated at the $Sal I$ site,it disrupts the $tet^R$ gene,leading to the loss of tetracycline resistance in the recombinant plasmid.

Ligation of foreign $\text{DNA}$ at which of the following site will result in loss of tetracycline resistance of $\text{pBR}^{322}$?

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