The significance of the heat shock method in bacterial transformation is that it facilitates:

What is the first step in the Southern Blotting technique?

Which of the following diagrams is correct for primer annealing in $PCR$?

To introduce recombinant $DNA$ into a host cell,it is first kept on ice,then placed at ........ temperature,and finally placed back on ice. (in $^{\circ} C$)

Identify the part of a bioreactor which is used as a foam breaker from the given figure.

Consider the following statements:
$I.$ In microinjection method,foreign $DNA$ is directly injected into the nucleus of an animal cell or plant cell by using microneedles or micropipettes.
$II.$ Microinjection method is used in oocytes,eggs,and embryos.
$III.$ Electroporation is the formation of temporary pores in the plasma membrane of a host cell by using lysozyme or calcium chloride.
$IV.$ In chemical-mediated gene transfer method,certain chemicals such as $CO_{2}$ help foreign $DNA$ to enter the host cell.
Which of the statements given above are correct?