How was insulin obtained before the advent of $rDNA$ technology? What were the problems encountered?
(N/A) Before the advent of $rDNA$ technology,insulin used for the treatment of diabetes was extracted from the pancreas of slaughtered cattle and pigs.
The major problem encountered was that insulin obtained from animal sources was not identical to human insulin.
Because it was a foreign protein,it caused some patients to develop allergies or other types of immune reactions.
The major problem encountered was that insulin obtained from animal sources was not identical to human insulin.
Because it was a foreign protein,it caused some patients to develop allergies or other types of immune reactions.
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View SolutionSome of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
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(vi) Extraction of recombinant gene product from $E. coli$.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.
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