(N/A) Restriction enzymes belong to a larger class of enzymes called nucleases.
$(i)$ Exonuclease enzymes: They remove nucleotides from the ends of the $DNA$.
$(ii)$ Endonuclease enzymes: Endonucleases make cuts at specific positions within the $DNA$.
Each restriction endonuclease functions by inspecting the length of a $DNA$ sequence. Once it finds the specific recognition sequence,it will bind to the $DNA$.
It then cuts each of the two strands of the double helix at specific points in their sugar-phosphate backbones.
- Each restriction endonuclease recognizes a specific palindromic nucleotide sequence in the $DNA$.
- The palindrome in $DNA$ is a sequence of base pairs that reads the same on the two strands when the orientation of reading is kept the same.