(N/A) The process of cloning and expressing a human gene in $E. coli$ involves the following steps:
$1$. Isolation of the target gene: The human gene encoding for growth hormone is isolated using restriction enzymes.
$2$. Selection of a vector: $A$ suitable cloning vector,such as a plasmid,is chosen.
$3$. Construction of recombinant $DNA$: The human gene is inserted into the vector using $DNA$ ligase to form recombinant $DNA$.
$4$. Transformation: The recombinant $DNA$ is introduced into the host bacterium,$E. coli$.
$5$. Selection and Screening: Transformed bacteria are selected using antibiotic resistance markers.
$6$. Expression: The $E. coli$ cells are cultured under optimal conditions to express the human growth hormone protein.