$A$ recombinant $DNA$ molecule was created by ligating a gene to a plasmid vector. By mistake,an exonuclease was added to the tube containing the recombinant $DNA$. How does this affect the next step in the experiment,i.e.,bacterial transformation?

Vedclass pdf generator app on play store
Vedclass iOS app on app store
(D) The experiment will not be affected because the recombinant $DNA$ molecule is a circular,closed structure with no free ends.
Exonucleases are enzymes that remove nucleotides specifically from the free ends of $DNA$ molecules.
Since the recombinant plasmid $DNA$ lacks free $3'$ or $5'$ ends,it will not act as a substrate for the exonuclease enzyme.
Therefore,the integrity of the recombinant $DNA$ remains intact,and the bacterial transformation process can proceed as planned.

Explore More

Similar Questions

Stirred-tank bioreactors have been designed for:

Consider the following figure of a stirred-tank bioreactor and identify $A, B, C$,and $D$.

What is the criterion for the movement of $DNA$ fragments on an agarose gel during gel electrophoresis?

It is used to detect $HIV$ in suspected $AIDS$ patient and detect mutations in genes in suspected cancer patient too.

$DNA$ can be introduced into any cell by:

Vedclass Products

For Students

Vedclass Test Series

Mock tests in real JEE/NEET style with performance analysis. 5-day free trial.

Start Free Trial
For Teachers

Exam Paper Generator

Generate Set A/B/C/D exam papers from 7.5L+ questions in 2 minutes. 3 chapters free.

Try Free
For Institutes

Online Exam Module

Live online exams with unlimited students, 360° analytics & white-label branding.

See Demo