Which of the following statements is consiste | vedclass

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(B) Plant tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,controlled environment. $1$. Sterilization is typically

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Maintain the explant at a temperature of $24^{\circ}C$.

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(B) Plant tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,controlled environment. $1$. Sterilization is typically achieved using an autoclave (high pressure and steam) or chemical disinfectants,not infrared light. $2$. The temperature for incubation is generally maintained between $20^{\circ}C$ to $28^{\circ}C$,making $24^{\circ}C$ a suitable and consistent condition for the growth of the explant. $3$. Explants are usually placed on a solid or semi-solid nutrient medium (agar-based) rather than just a liquid medium to provide structural support. $4$. While a quiet environment is good,it is not a technical requirement for the success of the culture process. Therefore,maintaining the explant at $24^{\circ}C$ is a standard practice in tissue culture.

Which of the following statements is consistent with the process of plant tissue culture?

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