In the PCR technique,how are the two strands | vedclass

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(A) The $PCR$ (Polymerase Chain Reaction) technique consists of three main steps: denaturation,annealing,and extension.In the denaturation step,the do

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By high temperature

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(A) The $PCR$ (Polymerase Chain Reaction) technique consists of three main steps: denaturation,annealing,and extension.In the denaturation step,the double-stranded $DNA$ is heated to a high temperature (approximately $94^{\circ}C - 96^{\circ}C$).This high temperature breaks the hydrogen bonds between the complementary base pairs,causing the two strands of $DNA$ to separate.Unlike in vivo replication,where helicase is used to unwind $DNA$,$PCR$ uses thermal denaturation to separate the strands.

In the $PCR$ technique,how are the two strands of $DNA$ separated?

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