A: Production of hirudin from transgenic Bras | vedclass

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(A) The assertion is correct because hirudin is an anticoagulant protein originally found in leeches. When scientists attempted to produce it in trans

Vedclass · Accepted Answer

Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

Vedclass · Answer

(A) The assertion is correct because hirudin is an anticoagulant protein originally found in leeches. When scientists attempted to produce it in transgenic $Brassica$ (mustard) plants,they used a synthetic gene because the natural eukaryotic gene contains introns.The reason is also correct because eukaryotic genes contain non-coding sequences called introns,which must be removed (spliced) to produce functional mRNA. Bacteria and many plant expression systems cannot efficiently process these introns. Therefore,to express the protein in $Brassica$,a synthetic gene (cDNA) lacking introns was designed and inserted.Since the presence of introns in the natural gene is the specific reason why a synthetic gene was required for successful expression,the Reason correctly explains the Assertion.

$A$: Production of hirudin from transgenic $Brassica$ required the use of a synthetic gene.
$R$: Eukaryotic genes have intron sequences that need to be spliced out.

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$A$: Production of hirudin from transgenic $Brassica$ required the use of a synthetic gene.$R$: Eukaryotic genes have intron sequences that need to be spliced out.