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| Column-$I$ | Column-$II$ |
|---|---|
| $(A)$ $\text{YAC}$ | $(I)$ Separation of $\text{DNA}$ fragments |
| $(B)$ $\text{Hind II}$ | $(II)$ Yeast |
| $(C)$ Gel electrophoresis | $(III)$ First restriction endonuclease |
| $(D)$ Heterologous host | $(IV)$ Recombinant protein |
| $I$ | $II$ | $III$ |
| $(a)$ $PCR$ | $(i)$ Specific base sequence | $p.$ $Taq$ polymerase |
| $(b)$ Downstream processing | $(ii)$ Inheritable gene | $q.$ Ampicillin resistant |
| $(c)$ Restriction endonuclease | $(iii)$ Amplification of $DNA$ | $r.$ $E. coli$ |
| $(iv)$ Product separation and modification | $s.$ Quality control |
| List-$I$ | List-$II$ |
| $A$. Genetically modified organism | $I$. Agrobacterium tumefaciens |
| $B$. $DNA$ polymerase | $II$. Bt cotton |
| $C$. Ti plasmid | $III$. Thermus aquaticus |
| $D$. pBR322 | $IV$. Escherichia coli |
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