Which technique is used to prove that antibio | vedclass

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(A) The experiment used to prove that antibiotic-resistant bacterial mutants are pre-existing and not induced by the environment is the $Replica \ pla

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Replica plating

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(A) The experiment used to prove that antibiotic-resistant bacterial mutants are pre-existing and not induced by the environment is the $Replica \ plating$ technique. This experiment was performed by $Joshua \ Lederberg$ and $Esther \ Lederberg$. In this process,a master plate containing bacterial colonies is pressed onto a velvet-covered block and then transferred to a new plate containing an antibiotic (like streptomycin). The colonies that grow on the antibiotic plate correspond exactly to the positions of the colonies on the master plate,proving that the resistance was already present in the population before exposure to the antibiotic.

Which technique is used to prove that antibiotic-resistant bacterial mutants are pre-existing and not induced by the environment?

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