Would you choose an exonuclease while produci | vedclass

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(B) No,an exonuclease would not be chosen. Exonucleases act on the free ends of a linear $DNA$ molecule by removing nucleotides one by one. Instead of

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No,because it degrades the $DNA$ from the ends.

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(B) No,an exonuclease would not be chosen. Exonucleases act on the free ends of a linear $DNA$ molecule by removing nucleotides one by one. Instead of producing $DNA$ fragments with sticky ends suitable for ligation,an exonuclease would shorten or completely degrade the $DNA$ fragment containing the gene of interest. Furthermore,a circular plasmid (vector) lacks free ends,so an exonuclease would not be able to cut it at all. Therefore,restriction endonucleases are preferred as they cut $DNA$ at specific internal sites to produce sticky or blunt ends.

Column-$I$	Column-$II$
$(a)$ Ligase	$(i)$ It removes nucleotides from $DNA$ ends.
$(b)$ Endonuclease	$(ii)$ Connects parts of $DNA$.
$(c)$ Exonuclease	$(iii)$ Removes phosphates from $DNA$.
$(d)$ $DNA$ Polymerase	$(iv)$ It produces sticky ends in $DNA$.
	$(v)$ Useful for repeated amplification.

Would you choose an exonuclease while producing a recombinant $DNA$ molecule?

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