Who used heavy nitrogen N^{15} for proving th | vedclass

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(B) In $1958$,Matthew Meselson and Franklin Stahl conducted an experiment using $E. coli$ bacteria to demonstrate that $DNA$ replication is semiconser

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Meselson and Stahl

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(B) In $1958$,Matthew Meselson and Franklin Stahl conducted an experiment using $E. coli$ bacteria to demonstrate that $DNA$ replication is semiconservative. They grew $E. coli$ in a medium containing $N^{15}$ (a heavy isotope of nitrogen) for several generations,allowing the $N^{15}$ to be incorporated into the $DNA$ molecules. Subsequently,they transferred these bacteria to a medium containing $N^{14}$ (a lighter isotope) and observed the density of the $DNA$ after successive generations using cesium chloride $(CsCl)$ density gradient centrifugation. The results showed that the $DNA$ molecules were hybrids of $N^{14}$ and $N^{15}$,confirming the semiconservative model.

Who used heavy nitrogen $N^{15}$ for proving the semiconservative way of $DNA$ replication?

Similar Questions

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