While doing a PCR,the 'denaturation' step is | vedclass

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(B) The $PCR$ process relies on the denaturation step to separate the double-stranded $DNA$ into single strands at high temperatures (around $94-98^{\

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Primers will not anneal to the template $DNA$.

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(B) The $PCR$ process relies on the denaturation step to separate the double-stranded $DNA$ into single strands at high temperatures (around $94-98^{\circ}C$).If the denaturation step is missed,the $DNA$ remains double-stranded.Consequently,the primers cannot bind (anneal) to the complementary sequences on the template $DNA$.As a result,the $DNA$ polymerase cannot initiate the extension process,and no amplification of the target $DNA$ sequence will occur.

While doing a $PCR$,the 'denaturation' step is missed. What will be its effect on the process?

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