Which one of the following is the peak absorp | vedclass

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(D) Heterocyclic nitrogenous bases (purines and pyrimidines) present in $DNA$ and $RNA$ exhibit a characteristic absorption of ultraviolet $(UV)$ ligh

Vedclass · Accepted Answer

$260$

Vedclass · Answer

(D) Heterocyclic nitrogenous bases (purines and pyrimidines) present in $DNA$ and $RNA$ exhibit a characteristic absorption of ultraviolet $(UV)$ light.This absorption is primarily due to the conjugated double bond systems within the ring structures of these bases.The peak absorption for these nitrogenous bases occurs at approximately $260 \ nm$.This property is widely used in molecular biology for the quantification of nucleic acids using spectrophotometry.

List-$I$	List-$II$
$a$. Bacteriophage $\phi \times 174$	$i$. $48502$ base pairs
$b$. Bacteriophage lambda	$ii$. $5386$ nucleotides
$c$. Escherichia coli	$iii$. $3.3 \times 10^9$ base pairs
$d$. Haploid content of human $DNA$	$iv$. $4.6 \times 10^6$ base pairs

Which one of the following is the peak absorption wavelength of ultraviolet light by heterocyclic nitrogenous bases (in $nm$)?

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