Which one of the following equipments is essentially required for growing microbes on a large scale for the industrial production of enzymes?

The transfer of protein from electrophoretic gel to nitrocellulose membrane is known as

From the following tools/techniques of genetic engineering,identify those which are required for cloning a bacterial gene in animal cells and choose the correct option:
$I$. Endonuclease $II$. Ligase
$III$. $A. tumefaciens$ $IV$. Microinjection
$V$. Gene gun $VI$. Lysozyme
$VII$. Cellulase $VIII$. Electrophoresis

In gel electrophoresis,the separated $DNA$ fragments are visualized after staining the $DNA$ with $...A...$ followed by exposure to $...B...$. Here $A$ and $B$ refer to:

Which method is used to amplify a desired gene in large numbers?

In which of the following wells was undigested $DNA$ loaded?