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(D) Recombinant $DNA$ technology involves the insertion of a specific gene into a vector to create a recombinant molecule.$Plasmids$ and $Phages$ are

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$DNA$ polymerase $III$

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(D) Recombinant $DNA$ technology involves the insertion of a specific gene into a vector to create a recombinant molecule.$Plasmids$ and $Phages$ are commonly used as vectors to carry the foreign $DNA$ into the host cell.$Restriction$ enzymes are essential for cutting the $DNA$ at specific recognition sequences to create sticky or blunt ends.$DNA$ polymerase $III$ is the primary enzyme responsible for $DNA$ replication in prokaryotes (like $E. coli$). It is not typically used in the construction of recombinant $DNA$ molecules in vitro; instead,$DNA$ ligase is used to join the $DNA$ fragments,and sometimes $DNA$ polymerase $I$ or $Taq$ polymerase is used for specific applications like filling in ends or $PCR$ amplification.

Which of the following would not be used in preparing recombinant $DNA$?

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