Which of the following is/are considered as application$(s)$ of biotechnology?
$I$. Waste treatment
$II$. Energy production
$III$. Bioremediation
$IV$. Processed food
$V$. Genetically modified crops for agriculture
$VI$. Diagnostics
$VII$. Therapeutics
Choose the correct option.

Statement $I$: $\text{RNA}$ interference $\text{(RNAi)}$ takes place in all eukaryotic organisms as a method of cellular defense.
Statement $II$: $Bt$ toxin protein is converted into an active form of toxin due to the alkaline $pH$ of the gut of an insect.

The transfer of the streptomycin resistance gene from $Nicotiana$ $tabacum$ to $Nicotiana$ $sylvestris$ was achieved through which method?

Product of biotechnology is

Match the following columns:

Column-$I$	Column-$II$
$(a)$ Pest resistant plant	$(1)$ Transgenic cow
$(b)$ Golden rice	$(2)$ $C$-peptide
$(c)$ Proinsulin	$(3)$ High vitamin $A$ content
$(d)$ Rosie	$(4)$ $RNA$ interference

Read the following four statements $(1-4)$ and identify the two statements that contain mistakes:
$1.$ The first transgenic buffalo,Rosie,produced milk which was human alpha-lactalbumin enriched.
$2.$ Restriction enzymes are used in the isolation of $DNA$ from other macromolecules.
$3.$ Downstream processing is one of the steps of $R-DNA$ technology.
$4.$ Disarmed pathogen vectors are also used in the transfer of $R-DNA$ into the host.
Which are the two statements having mistakes?